T7 RiboMAX Express RNAi System from Promega

Product Specs
ItemT7 RiboMAX Express RNAi System
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP1700
Quantity50 x 20µl reactions
PromoterT7

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) 5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expressionNat CommunDecember 12, 2023Zheng Zhao, Liang Li, Ruichen Zeng, Liangguan Lin, Dongwei Yuan, Yejie Wen, Na Li, Yingying Cui, Shiming Zhu, Zhi-Min Zhang, et al.antisense RNA was synthesized in a single reaction using a T7 RiboMAX Express RNAi System (Promega, P1700). After purification, the double-stranded RNA (dsRNA) concentration was adjusted to 3 μg/μl15. Under

(2) Systematic assessment of transcriptomic and metabolic reprogramming by blue light exposure coupled with agingPNAS NexusDecember 5, 2023Jia Huang, Fan Zhou, Huanchan Zhou, Xiaoqi Zheng, Zhengyi Huo, Meng Yang, Zihe Xu, Runzhou Liu, Luoluo Wang, Xiaoyun WangSigma-Aldrich) at 27°C. RNAi Following the manufacturer's protocol, the T7 RiboMAX Express RNAi System (#P1700, Promega, WI, USA) was used for the synthesis of double-stranded (dsRNA) molecules. We also prepared

(3) Functional assessment of lysosomal Rab7 and RILP with RNA interference and overexpression in Spodoptera frugiperda Sf9 cell linesSTAR ProtocolsDecember 1, 2023Gaofeng Cui, Zhiyan Jiang, Guohua Zhongcommercial assays,Critical commercial assays ,, T7 RiboMAX Express RNAi System,"Promega, USA",Cat#P1700 FuGENE HD transfection reagent,"Promega, USA",Cat#E2311 E.Z.N.A. Endo-Free Plasmid Mini Kit II,"Omega,

(4) Functional characterization of alkaline phosphatases involved alarm pheromone in the vetch aphid Megoura viciaeiScienceNovember 17, 2023Xuan Song, Yao-Guo Qin, Yi-Han Zhang, Yu-Bei Zhou, Dan Chen, Dong-Hai Xie, Zheng-Xi Liinhibitors,Beyotime,P0013J Alkaline Phosphatase Assay Kit,Beyotime,P0321S T7 RiboMAX™ Express RNAi System,Promega,P1700 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Primers are listed in Table S1,This paper,N/A ,, Software

(5) 4-Vinylanisole promotes conspecific interaction and acquisition of gregarious behavior in the migratory locustProceedings of the National Academy of SciencesSeptember 12, 2023Jing Yang, Qiaoqiao Yu, Jia Yu, Le Kang, Xiaojiao Guofluorescent protein (GFP) and Or35 were synthesized by using the T7 RiboMAX Express RNAi system (Promega, WI, P1700) according to the manufacturer’s instructions. The fragments used in the experiment were blasted against

(6) Evolutional insights into the interaction between Rab7 and RILP in lysosome motilityiScienceJuly 21, 2023Gaofeng Cui, Zhiyan Jiang, Yaoyao Chen, Yun Li, Shupei Ai, Ranran Sun, Xin Yi, Guohua ZhongChina",CW0894S FuGENE® HD Transfection Reagent,"Promega, USA",E2311 T7 RiboMAX™ Express RNAi System,"Promega, USA",P1700 E.Z.N.A.® Endo-Free Plasmid Mini Kit II,"Omega, USA",D6950-01 Eastep® Super Total RNA Extraction Kit,"Promega,

(7) Damage sensing mediated by serine proteases Hayan and Persephone for Toll pathway activation in apoptosis-deficient fliesPLOS GeneticsJune 15, 2023Shotaro Nakano, Soshiro Kashio, Kei Nishimura, Asuka Takeishi, Hina Kosakamoto, Fumiaki Obata, Erina Kuranaga, Takahiro Chihara, Yoshio Yamauchi, Toshiaki Isobe, et al.in S2 cells, double-stranded RNAs (dsRNAs) were generated using the T7 RiboMAX Express RNAi System (P1700, Promega, U.S.A.) according to the manufacturer’s instructions. For the negative control dsRNA, pBlueScript

(8) Hypoxia-induced tracheal elasticity in vector beetle facilitates the loading of pinewood nematodeeLifeMarch 30, 2023Xuan Tang, Jiao Zhou, Tuuli-Marjaana Koski, Shiyao Liu, Lilin Zhao, Jianghua Sunon both ends (Supplementary file 2), according to the T7 RiboMax Express RNAi System Kit (Promega, P1700) protocol. Within 2 days after molting, approximately 60 adults beetle were injected at the joint of

(9) Apical PAR-3 caps orient the mitotic spindle in C. elegans early embryosbioRxivMarch 30, 2023Naomi J. Stolpner, Nadia I. Manzi, Thomas Su, Daniel J. Dickinsonpromoters. ssRNA was transcribed from this PCR product using the Promega T7 RiboMAX Express kit (Cat. No. P1700), purified, and annealed to form dsRNA. dsRNA was injected into the body of young adult worms at 1μg/μL

(10) BmCBP Catalyzes the Acetylation of BmApoLp-II Protein and Regulates Its Stability in Silkworm, Bombyx moriInsectsMarch 23, 2023Chen Y, Lv J, Zu G, Yang F, Geng J, You Z, Jiang C, Sheng Q, Nie Z.pTA-BmCBP as a template. Then the linear DNA and T7 RiboMAX™ Express RNAi System (Promega, Madison, USA, P1700) was used to synthesize the dsRNA specific to a particular target region of the BmCBP. The primers used

(11) A novel gene REPTOR2 activates the autophagic degradation of wing disc in pea aphideLifeMarch 21, 2023Erliang Yuan, Huijuan Guo, Weiyao Chen, Bingru Du, Yingjie Mi, Zhaorui Qi, Yiyang Yuan, Keyan Zhu-Salzman, Feng Ge, Yucheng Suncontrol. All chemicals were administered via 24-hr feeding. The T7 RiboMAX Express RNAi System (Promega, P1700) was used to synthesize double-stranded RNA (dsRNA) according to the manufacturer’s protocol. dsRNAs,

(12) Arboviruses and symbiotic viruses cooperatively hijack insect sperm-specific proteins for paternal transmissionNature CommunicationsMarch 9, 2023Jiajia Wan, Qifu Liang, Ruonan Zhang, Yu Cheng, Xin Wang, Hui Wang, Jieting Zhang, Dongsheng Jia, Yu Du, Wenhui Zheng, et al.(dsP8), or GFP (dsGFP) according to the protocol for the T7 RiboMAX Express RNAi System kit (Promega, P1700). To test the knockdown of HongrES1 expression on RdFV or RGDV infection in male reproductive systems,

(13) Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid ShrimpMarine DrugsFebruary 28, 2023Zishu Huang, Xiaoyu Zheng, Zeyan Chen, Zhihong Zheng, Defu Yao, Shen Yang, Yueling Zhang, Jude Juventus Aweya(GenBank: UDY80669) designated dsEGFP were synthesized using the T7 RiboMAXTM Express RNAi System kit (Cat# P1700, Promega, Madison, WI, USA). The primer sequences used for the in vitro dsRNA synthesis are shown in

(14) Signaling events induced by lipopolysaccharide-activated Toll in response to bacterial infection in shrimpFrontiers in ImmunologyFebruary 3, 2023Sheng Wang, Haoyang Li, Qinyao Li, Bin Yin, Sedong Li, Jianguo He, Chaozheng Liwere synthesized by in vitro transcription with T7 RiboMAX Express RNAi System kit (Promega, cat. no. P1700) ( Supplementary Table 1 ). The dsRNAs targeting Toll1/2/3/4/5/6/7/8/9, TAK1, and TRAF6 were obtained

(15) Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profilesProceedings of the National Academy of SciencesJanuary 31, 2023Jing Li, Liya Wei, Yongsheng Wang, Haikang Zhang, Pengcheng Yang, Zhangwu Zhao, Le KangCo. RNAi. Double-stranded RNAs were synthesized with the T7 RiboMAX™ Express RNAi System (Promega, P1700) according to the instructions in the kit manual. The primers used for RNAi are listed in the supplemental

(16) Southern rice black-streaked dwarf virus induces incomplete autophagy for persistence in gut epithelial cells of its vector insectPLoS pathogensJanuary 1, 2023Zhang L, Liu W, Wu N, Wang H, Zhang Z, Liu Y, Wang X.replaced with Sf-900 III medium. RNA interference assay The T7 Ribomax Express RNAi System (Promega, P1700) was used to synthesize dsRNAs. Each treatment group included 200 viruliferous third-instar WBPH nymphs,

(17) A leafhopper saliva protein mediates horizontal transmission of viral pathogens from insect vectors into rice phloemCommunications BiologyDecember 1, 2022Wei Wu, Ge Yi, Xinwei Lv, Qianzhuo Mao, Taiyun Weiregions of RdCBP or GFP were synthesized in vitro using the T7 RiboMAX Express RNAi System (Promega Cat#:P1700). The 50-s instar nymphs of R. dorsalis were microinjected with 30 nl of a mixture of purified viruses

(18) Search performance and octopamine neuronal signaling mediate parasitoid induced changes in Drosophila oviposition behaviorNature CommunicationsDecember 1, 2022Lan Pang, Zhiguo Liu, Jiani Chen, Zhi Dong, Sicong Zhou, Qichao Zhang, Yueqi Lu, Yifeng Sheng, Xuexin Chen, Jianhua Huangmicroinjection Double-stranded RNA (dsRNA) was synthesized using the T7 RiboMAX Express RNAi System Kit (Promega, Cat# P1700) according to the manufacturer’s instructions. The primers used, which are complementary to the Lb Orco

(19) Transcriptional Activation of Ecdysone-Responsive Genes Requires H3K27 Acetylation at EnhancersInternational Journal of Molecular SciencesSeptember 16, 2022Dong Cheng, Zhaoming Dong, Ping Lin, Guanwang Shen, Qingyou XiaBmCBP RNAi, double-stranded RNAs (dsRNAs) were synthesized using the T7 RiboMAX Express RNAi System (P1700; Promega, Madison, WI, USA), following the manufacturer’s instructions, with EGFP dsRNA as the control.

(20) The modulatory effects of biogenic amines on male mating performance in Bactrocera dorsalisFrontiers in PhysiologySeptember 6, 2022Wenlong Chen, Yaoyao Chen, Ziwei Xiao, Yuhua Zhang, Tong Zhang, Guohua Zhong, Xin Yiand the enzyme digestion product was used to synthesize dsRNA with a T7 RiboMAX Express RNAi System (P1700, Promega, United States). Simultaneously, green fluorescent protein (GFP) was used as a control. The

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